Journal: Stem Cell Research & Therapy

Article Title: Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease

doi: 10.1186/s13287-015-0095-0

Figure Lengend Snippet: The expression of E-cadherin and α-SMA in HK2 cells. a The analysis of gene expression of E-cadherin and a-SMA in HK2 cells following TGF-β1 (6 ng/ml) by RT-PCR at two time points (48 and 72 h) with MVs or EPO-MVs. All of the experiments were repeated three times (n = 3). b Western blot was used to detect the protein expression of E-cadherin and α-SMA in HK2 cells following TGF-β1 (6 ng/ml) at two time points (48 and 72 h) with MVs or EPO-MVs. All of the experiments were repeated three times (n = 3). *p < 0.01, 48-h TGF-β1 group versus HK-2 group or 72-h TGF-β1 group versus HK-2 group, $ p < 0.01, 48-h HK2 + TGF-β1 + MV group versus TGF-β1 group or 72-h HK2 + TGF-β1 + MV group versus TGF-β1 group, # p < 0.05, 48-h HK2 + TGF-β1 + EPO-MV group versus HK2 + TGF-β1 + MV group or 72-h HK2 + TGF-β1 + EPO-MV group versus HK2 + TGF-β1 + MV. EPO erythropoietin HK2 human kidney 2 MVs microvesicles TGF -β1 transforming growth factor-β1 α-SMA α-smooth muscle actin

Article Snippet: Total RNA was extracted from MVs and HK2 cells according to the Trizol (Invitrogen) manufacturer’s protocol. cDNA was synthesized using the Takara SYBR®PrimeScriptTM miRNA RT-PCR Kit and the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Shiga, Japan).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: The Hierarchical Cluster Analysis of differentially expressed protein-coding transcripts ( A ), miRNAs ( B ), and lncRNA transcripts ( C ).

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques:

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: Overview of small RNA sequencing in the chicken ovary. ( A ) Portions of small RNA types in the clean reads. The percent of miRNA is approximately 73%, and the other 28% included rRNA, scRNA, snRNA, snoRNA, tRNA, simple repeats, exon sense/antisense and intron sense/antisense. ( B ) Relative frequency of different types of small RNAs in the atrophic ovary (AO) and normal ovary (NO). ( C ) Size distribution of all miRNAs. The X-axis depicts their length (nt), and the Y-axis represents frequency (%). ( D ) The relative proportion of the top 10 miRNAs in the total amount of miRNA.

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques: RNA Sequencing

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: Sixteen intersection genes and their corresponding pathways and differentially expressed miRNAs * .

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques:

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: The miRNA-gene-pathway network between sixteen intersection genes, and their corresponding pathways and differentially expressed miRNAs. Hexagon, round rectangle and ellipse indicate pathway, gene and miRNA, respectively. Red and green mean up- and down-regulation, respectively.

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques:

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: An overview of the competing endogenous RNA (ceRNA) network. Rectangle, ellipse and V indicate miRNA, protein-coding transcript and lncRNA transcript, respectively. Green and red indicate down- and up-regulation, respectively.

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques: